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  • O identify the best marker for alveolar soft part sarcoma in formalin-fixed, paraffin-embedded tissues, we evaluated the sensitivity and specificity of the detection of the aspscr1-tfe3 fusion transcript along with the immunoreactivity for tfe3 and cd147 in 24 alveolar soft part sarcomas and 23 non–alveolar soft part sarcoma tumors, including 5 granular cell tumors, 5 paragangliomas, 3 clear cell sarcomas, and 10 clear cell renal cell carcinomas. The aspscr1-tfe3 fusion transcript was detected in 24 of 24 alveolar soft part sarcomas (7 type 1, 17 type 2), and tfe3 immunoreactivity was observed in 22 of 24 alveolar soft part sarcomas. In non–alveolar soft part sarcoma tumors, the aspscr1-tfe3 fusion transcript was not detected; however, the tfe3 immunoreactivity was observed in 2 of 5 granular cell tumors. Cd147 immunoreactivity was demonstrated in 20 of 24 alveolar soft part sarcomas, 3 of 5 granular cell tumors, and 8 of 10 clear cell renal cell carcinomas. Our results demonstrate that the most sensitive marker of alveolar soft part sarcoma was the presence of the aspscr1-tfe3 fusion transcript. Thus, detection of the aspscr1-tfe3 fusion transcript was considered applicable for formalin-fixed, paraffin-embedded tissues with superior sensitivity as compared with tfe3 immunohistochemical staining. In alveolar soft part sarcomas with unusual locations or histology, we consider that the detection of the aspscr1-tfe3 fusion transcript would be the highly effective diagnostic technique. Abbreviations asps, alveolar soft part sarcoma; rcc, renal cell carcinoma; rt-pcr, reverse transcription polymerase chain reaction keywords alveolar soft part sarcoma; differential diagnosis; aspscr1-tfe3 fusion transcript; rt-pcr; immunohistochemistry figures and tables from this article: fig. 1.  schematic diagram of the aspscr1-tfe3 fusion product. Sequences (105 bp long) are only found in the type 2 fusion product. Relative dimensions are approximate. Arrows indicate first (upper part) or nested (lower part) primers. viagra without a doctor prescription generic viagra online cheap viagra online cheap generic viagra viagra online cheap generic viagra viagra online buy generic viagra viagra for sale viagra for sale Figure options view in workspace fig. Diseño Multi-dispositivo
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